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    6. 紅鶴頂蘭遠緣雜交及花梗培養

    紅鶴頂蘭遠緣雜交及花梗培養

    Author 蔡雅竹
    Publisher 華藝數位 /蔡雅竹
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    Released
    2021/12/15
    Language
    Traditional Chinese
    File format
    PDF (5MB), fit in large screen and Pubook
    Pages
    120
    ID
    313770
    ISBN
    ABK1100000027
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    紅鶴頂蘭(Phaius tankervilleae)為台灣原生地生蘭,花莖長,花數多達10-20朵花,花被外側白色,內側紫紅色。為建立紅鶴頂蘭的花梗培養方法,以紅鶴頂蘭花梗之頂端花芽、花芽及花梗營養芽做為初代培養之培植體,小於5 cm的花梗在花梗頂芽有最高芽體形成率為66.7%。10-15 cm的花梗在花芽及花梗營養芽的芽體形成率最高,分別為7.1%及10.9%。將源自於紅鶴頂蘭花梗花芽及源自花梗營養芽的培植體在增殖後每個月可增生1.3-1.9倍的芽體,移入發根培養基2個月後移植出瓶,以花梗花芽為培植體的小苗最高可得1條根,葉最長為2.9 cm。小苗在移至溫室栽培兩個月後,以源自花梗花芽為培植體之小苗株高最高為3.5 cm,存活率最高為87.5%。
    本研究收集紅鶴頂蘭近緣屬包含竹葉蘭屬(Arundina)、白及屬(Bletilla)、根節蘭屬(Calanthe)、蕙蘭屬(Cymbidium)、苞舌蘭屬(Spathoglottis)及兩種人工雜交屬:囊唇鶴頂蘭屬(Gastrophaius)及根節鶴頂蘭屬(Phaiocalanthe)進行染色體數、花粉體內外發芽及雜交試驗。紅鶴頂蘭的染色體數為2n= ca. 46,其近緣屬的染色體數大部分在2n= 40 左右,紅鶴頂蘭花粉體外培養在蔗糖濃度10%的BK培養基,於25℃下培養生長較佳,培養十天後發芽率可至24.2%。
    紅鶴頂蘭雜交之蒴果大部分在授粉後4至6週發育漸緩,透過植體內花粉管觀察可知以紅鶴頂蘭為父本時,花粉管生長良好,然而,花粉體外發芽率與雜交親合性並無一定的關連性,但花粉活力低、花粉生長受抑制或母本發育不全會導致紅鶴頂蘭遠緣雜交之組合無法結實。
    紅鶴頂蘭遠緣雜交共計有11種組合與9種異屬植物雜交後可得雜交後代,當紅鶴頂蘭為母本時,可與Cal. discolor、黃根節蘭、三板根節蘭及Cym. Golden Rush x Cym. Golden Birds雜交後獲得子代,雜交成功的比例的占44.4%,當紅鶴頂蘭為父本時,與蘭嶼白及、Cal. discolor、玉花四季蘭、Gastrophaius Queen Margrethe、紫苞舌蘭、Spa. plicata form. alba、Spa. plicata form. pink雜交也已獲得雜交後代,將紫苞舌蘭、Spa. plicata form. alba、Spa. plicata form. pink視為同種時,以紅鶴頂蘭為父本雜交成功的比例占55.5%。
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    Released
    2021/12/15
    Language
    Traditional Chinese
    File format
    PDF: Fit in large screen
    Provide Adobe DRM
    Provide PDF
    Offer DRM free license
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    ID
    313770
    ISBN
    ABK1100000027

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